![]() Salivary melatonin was measured using radioimmunoassay the times of evening onset and morning offset were objectively determined using the hockey-stick algorithm and served as circadian phase markers. They collected 19 saliva samples at home over 24 h (including 2 samples during the night) on July 3-4, and December 18-19, 2015. ![]() during their regular 5-workdays schedule. Sixty-three volunteers entered the study, and 46 completed it. The study aimed to quantify a seasonal change in circadian rhythms and its relationship to the social/sleep regimen in humans living in Novosibirsk (55°N), using the naturalistic situation that daylight saving time transitions have been abolished in Russia.
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